pIncW-T7 RNAP plasmid原核表达质粒IPTG诱导表达T7 RNAP质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIncW-T7 RNAP plasmid原核表达质粒IPTG诱导表达T7 RNAP质粒

expresses an attenuated T7 RNAP under an isopropyl 尾-d-1-thiogalactopyranoside (IPTG) inducible control

Vector typeBacterial Expression, Synthetic Biology

Bacterial Resistance(s)Spectinomycin
Growth Temperature37°C
Growth Strain(s)DH10B
Growth instructionsPlasmid is maintained at only 2-3 copies/cells, so it is difficult to sequence from minipreps (better to use PCR).
Copy numberLow Copy

Gene/Insert name T7 RNAP*
Alt nameattenuated T7 RNAP
SpeciesBacteriophage T7
MutationR632S
Promoterp Tac-symmetric
Tag / Fusion Protein
umuD degradation tag (N terminal on backbone)

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net