pGP-CMV-jGCaMP7f plasmid vector超敏感钙离子指示器蛋白表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pGP-CMV-jGCaMP7f plasmid vector超敏感钙离子指示器蛋白表达质粒载体

Mammalian expression of ultrasensitive protein calcium sensor

Vector backbone pCMV
Total vector size (bp)5308
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert namejGCaMP7f
Alt nameGCaMP3-T302L R303P A317L D380Y
Alt nameGCaMP3 variant 921
Alt nameJanelia GCaMP7
SpeciesR. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
Insert Size (bp)1353
PromoterCMV
Tag / Fusion Protein
T7 epitope, Xpress tag, 6xHis
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBglII (not destroyed)
3′ cloning siteNotI (not destroyed)
5′ sequencing primerATGGGAGTTTGTTTTGGC

Supplier来源:BioVector NTCC Inc.
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Website网址: http://www.biovector.net