HPD- 2NR cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HPD- 2NR cell line细胞株

Cell line: HPD-2NR
DSMZ no.: ACC 293
Species validation: Syrian hamster (golden hamster) (Mesocricetus auratus)
Cell type: pancreas ductal adenocarcinoma
Origin: established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-1NR (DSM ACC 314); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene
Reference(s): 14784
Biosafety level: 1
Permissions and restrictions: A
 DSMZ Cell Culture Data:
Morphology: adherent, epithelial cells growing as monolayers
Medium: 80% RPMI 1640 + 20% h.i. FBS
Subculture: split confluent culture 1:3 to 1:8 every 2-6 days using trypsin/EDTA; seed out at about 0.4 x 105 cells/cm2
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 40 hours
Harvest: cell harvest of ca. 1.5-2.0 x 105 cells/cm2
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
 DSMZ Scientific Data:
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Species validation: Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species.
Cytogenetics: near triploid highly rearranged karyotype with 29% polyploidy - 58-67<3n>XX, -X - hamster markers present - resembles published karyotype
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net