RPMI-1846 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

RPMI-1846 cell line细胞株

Cell Line Name: RPMI 1846
Other Collection No.: ATCC CCL 49，(ECACC 90102527)
Keywords: Hamster Golden Syrian melanotic melanoma
Cell Line Description: Derived from a spontaneous malignant melanoma in an aged golden syrian hamster. The cells have the ability to produce melanin in vitro.
Species: Hamster
Tissue of Origin: skin
CellType: Epithelial
Growth Mode: Adherent
Karyotype: 2n = 44

Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin; 5% CO2; 37°C.
Culture Medium: McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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