BL21ΔABCF strain大肠杆菌基因敲除菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

BL21ΔABCF strain大肠杆菌基因敲除菌株/感受态细胞

Genotype = ΔompA ΔompC ΔompF ΔlamB
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]

Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene.

Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.

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