C3040 E.coli strain大肠杆菌宿主菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

C3040 E.coli strain大肠杆菌宿主菌株/感受态细胞

Description:
E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.

Highlights
Recommended host strain for cloning genes into retroviral/ lentiviral vectors
Recommended for cloning of direct repeats and inverted repeat sequences
Reduced recombination of cloned DNA (recA1)
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening with vectors capable of alpha-complementation

Genotype
F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR)/ ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC)

Features
High quality plasmid preparations due to endA mutation
T1 phage resistance (fhuA)
Value pricing
Free of animal products

Application Features
Cloning unstable inserts
Isolating and propagating retroviral/ lentiviral clones
Compatible with NEBuilder HiFi DNA Assembly, Gibson Assembly reactions as well as ligation reactions


Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net