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HUDEP-2 Cell Line RCB4557人永生化红细胞系-BioVector NTCC典型培养物保藏中心

  • 价  格:¥598685
  • 货  号:RCB4557
  • 产  地:北京
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Name:HUDEP-2人永生化红细胞系

Cat# BioVector924733

Comment

Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.


Basic information



Year of deposit

2013

Cloning (depositor)

No

Animal

human < Mammals

Genus

Homo

Species

sapiens

Race

Japanese

Tissue

umbilical cord blood

Classification

transformed

Recombinant

recombinant

Exogene

KUSABIRA-ORANGE, HPV16 E6/E7

Vector

CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector


Lifespan

infinite

Morphology

lymphocyte-like




deposit info

lot info


Medium

Medium List


Culture type

Suspension cells

Suspension cells

Medium and additives

StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml)

StemSpan SFEM +3U/ml EPO + 50ng/ml SCF + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline

Antibiotics


Free


Passage method


dilution

Culture information

Passage ratio


1 : 5-10 split

SC frequency

Subculture : 2 times/week

Subculture : 2 times/week

Temperature

37 ℃

37 ℃

CO2 concentration

5 %

5 %

Freeze medium

Medium + 10% DMSO

CELLBANKER 1

Freezing method


Slow freezing

Mycoplasma


(-)

Animal PCR


OK

Virus (HIV)


Undetected

Virus (HTLV-1)


(-)

Description描述:Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.
ID编号: NTCC-RCB4557
Size/Quantity数量:1 vial
Biosafety Level生物安全级别: 1
Shipping Info运输方式: Dry Ice
Storage储存方式: Liquid Nitrogen
Species物种:
Gender性别:
Product Type细胞类型:
Images图片:
Complete Growth Medium完全培养基:
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
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