HUDEP-2 Cell Line RCB4557人永生化红细胞系-BioVector NTCC典型培养物保藏中心
- 价 格:¥598685
- 货 号:RCB4557
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
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手机:18901268599
地址:北京
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Name:HUDEP-2人永生化红细胞系
Cat# BioVector924733
Comment | Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin. | |||
Basic information | ||||
Year of deposit | 2013 | |||
Cloning (depositor) | No | |||
Animal | human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Race | Japanese | |||
Tissue | umbilical cord blood | |||
Classification | transformed | |||
Recombinant | recombinant | |||
Exogene | KUSABIRA-ORANGE, HPV16 E6/E7 | |||
Vector | CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector | |||
Lifespan | infinite | |||
Morphology | lymphocyte-like | |||
deposit info | lot info | |||
Medium | Medium List | |||
Culture type | Suspension cells | Suspension cells | ||
Medium and additives | StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml) | StemSpan SFEM +3U/ml EPO + 50ng/ml SCF + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline | ||
Antibiotics | Free | |||
Passage method | dilution | |||
Culture information | Passage ratio | 1 : 5-10 split | ||
SC frequency | Subculture : 2 times/week | Subculture : 2 times/week | ||
Temperature | 37 ℃ | 37 ℃ | ||
CO2 concentration | 5 % | 5 % | ||
Freeze medium | Medium + 10% DMSO | CELLBANKER 1 | ||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Animal PCR | OK | |||
Virus (HIV) | Undetected | |||
Virus (HTLV-1) | (-) |
Description描述:Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.
ID编号: NTCC-RCB4557
Size/Quantity数量:1 vial
Biosafety Level生物安全级别: 1
Shipping Info运输方式: Dry Ice
Storage储存方式: Liquid Nitrogen
Species物种:
Gender性别:
Product Type细胞类型:
Images图片:
Complete Growth Medium完全培养基:
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:
Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话:400-800-2947
Email:Biovector@163.com
http://www.biovector.net
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