HeLa-Difluo hLC3 Autophagy Reporter cells 细胞株-BioVector NTCC典型培养物保藏中心

Name：HeLa-Difluo hLC3 Autophagy Reporter cells
Description描述：Monitoring autophagy activity HeLa-Difluo™ hLC3 reporter cells are designed to monitor the autophagic flux by means of a fluorescent-labeled LC3B protein. These cells, derived from the HeLa human epithelial carcinoma cell line, express the RFP::GFP::LC3 fusion protein where the human LC3B (microtubule-associated protein 1 light chain 3 beta) is fused to two fluorescent reporter proteins: RFP (acid-stable) and GFP (acid-sensitive). The LC3B protein is a good marker to assess the autophagic flux since it is recruited from the cytosol to be associated to all the autophagic structures: the nascent autophagosome (called the isolation membrane), the complete autophagosome and the autolysosome. Upon autophagy induction, diffuse cytoplasmic fluorescence shifts to autophagosomes and autolysosomes as fluorescent puncta. Monitoring of autophagy activity relies on microscopy visualization of fluorescent puncta, progressive degradation of the GFP, and concurrent increase of RFP signal in autolysosomes. The number of fluorescent punctate structures per cell, the percentages of RFP-GFP positive and RFP positive cells can be used to assess autophagic flux as described previously [1,2]. HeLa-Difluo™ hLC3 cells are resistant to Zeocin®. References 1. Loos B. et al. 2014. Defining and measuring autophagosome flux- concept and reality. Autophagy. 2014;10(11):2087-96. 2. Kimura s. et al., 2007. Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescenttagged LC3. Autophagy. 3(5):452-60. Figures Detection of fluorescent puncta from rapamycin-mediated autophagy in Hela-Difluo cellsDetection of fluorescent puncta from rapamycin-mediated autophagy Back to the top Specifications Antibiotic resistance: Zeocin® Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™. Quality control: HeLa-Difluo™ hLC3 cells have been tested for their ability to respond to autophagic inducers. The stability of this cell line for 20 passages following thawing has been verified. HeLa-Difluo™ hLC3 cells are guaranteed mycoplasma-free.
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Size/Quantity数量:1 vial
Biosafety Level生物安全级别: 1
Shipping Info运输方式: Dry Ice
Storage储存方式: Liquid Nitrogen
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Product Type细胞类型:
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Complete Growth Medium完全培养基:
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
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