J774-Dual Murine NF-kB & IRF Reporter macrophage-like cells细胞株-BioVector NTCC典型培养物保藏中心
- 价 格：¥98965
- 货 号：J774-Dual Murine NF-kB & IRF Reporter macrophage-like cells
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Name：J774-Dual Murine NF-kB & IRF Reporter macrophage-like cells
Description描述：NF-kB-SEAP & IRF-Luc Reporter Macrophages J774-Dual™ cells have been derived from the mouse J774.1 macrophage-like cell line by stable integration of two inducible reporter constructs. J774-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site. J774-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, J774-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™, a Lucia luciferase detection reagent. Figures NF-κB Reponse of J774-Dual cells to PRR ligandsNF-κB Reponse of J774-Dual cells to PRR ligands IRF Response of J774-Dual cells to various PRR ligandsIRF Response of J774-Dual cells to various PRR ligands Back to the top Specifications Antibiotic resistance: Blasticidin and Zeocin® Guaranteed mycoplasma-free Quality Control: For each lot, proper activation of the NF-κB pathway and IRF pathway is confirmed upon stimulation of J774-Dual™ cells by various pathogen associated molecular patterns (PAMPs) known to activate these pathways. The stability of this cell line for 20 passages following thawing has been verified. These products are covered by a Limited Use License (See Terms and Conditions). Back to the top Contents 1 vial of J774-Dual™ cells (3-7 x 106 cells) 1ml of Blasticidin (10 mg/ml) 1 ml of Zeocin® (100 mg/ml) 1 ml of& Normocin™ (50 mg/ml) 1 pouch of QUANTI-Luc™ 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent) Shipped on dry ice Shipped on dry ice (Europe, USA & Canada) Back to the top Details J774.1 cells express a variety of pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) [1, 2], C-type lectin receptors (CLRs) [2,3], RIG-I-like receptors (RLRs) [4]. Upon recognition of their cognate PAMPs, these receptors induce signaling pathways leading to the activation of the transcription factors NF-kB and/or IRF3/7. Stimulation of J774-Dual™ cells with the following PAMPs, Pam3CSK4 (TLR1/2), lipopolisaccharide (TLR4), CpG ODNs (TLR9), L18-MDP (NOD2) and TDB (Mincle), leads to the activation of NF-kB. Stimulation with RLR ligands, such as transfected poly(I:C) or 5’ppp-dsRNA, or the STING agonist, 2’3’-cGAMP, triggers the IRF pathway. J774-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin®. 1. Jin M. et al., 2011. Effects of chondroitin sulfate and its oligosaccharides on toll-like receptor-mediated IL-6 secretion by macrophage-like J774.1 cells. Biosci Biotechnol Biochem. 75(7):1283-9. 2. Kushida T. et al., 2011. Enhancement of Dectin-2 gene expression by lignin-carbohydrate complex from Lentinus edodes mycelia extract (LEM) in a mouse macrophage-like cell line. Anticancer Res. 31(4):1241-8. 3. Takeda Y. et al., 2007. Ternary complex consisting of DNA, polycation, and a natural polysaccharide of schizophyllan to induce cellular uptake by antigen presenting cells. Biomacromolecules. 8(4):1178-86. 4. Wilden H. et al., 2009. Expression of RIG-I, IRF3, IFN-beta and IRF7 determines resistance or susceptibility of cells to infection by Newcastle Disease Virus. Int J Oncol. 34(4):971-82.
ID编号:
Size/Quantity数量:1 vial
Biosafety Level生物安全级别: 1
Shipping Info运输方式: Dry Ice
Storage储存方式: Liquid Nitrogen
Species物种:
Gender性别:
Product Type细胞类型:
Images图片:
Complete Growth Medium完全培养基:
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:
Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net
Description描述：NF-kB-SEAP & IRF-Luc Reporter Macrophages J774-Dual™ cells have been derived from the mouse J774.1 macrophage-like cell line by stable integration of two inducible reporter constructs. J774-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site. J774-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, J774-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™, a Lucia luciferase detection reagent. Figures NF-κB Reponse of J774-Dual cells to PRR ligandsNF-κB Reponse of J774-Dual cells to PRR ligands IRF Response of J774-Dual cells to various PRR ligandsIRF Response of J774-Dual cells to various PRR ligands Back to the top Specifications Antibiotic resistance: Blasticidin and Zeocin® Guaranteed mycoplasma-free Quality Control: For each lot, proper activation of the NF-κB pathway and IRF pathway is confirmed upon stimulation of J774-Dual™ cells by various pathogen associated molecular patterns (PAMPs) known to activate these pathways. The stability of this cell line for 20 passages following thawing has been verified. These products are covered by a Limited Use License (See Terms and Conditions). Back to the top Contents 1 vial of J774-Dual™ cells (3-7 x 106 cells) 1ml of Blasticidin (10 mg/ml) 1 ml of Zeocin® (100 mg/ml) 1 ml of& Normocin™ (50 mg/ml) 1 pouch of QUANTI-Luc™ 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent) Shipped on dry ice Shipped on dry ice (Europe, USA & Canada) Back to the top Details J774.1 cells express a variety of pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) [1, 2], C-type lectin receptors (CLRs) [2,3], RIG-I-like receptors (RLRs) [4]. Upon recognition of their cognate PAMPs, these receptors induce signaling pathways leading to the activation of the transcription factors NF-kB and/or IRF3/7. Stimulation of J774-Dual™ cells with the following PAMPs, Pam3CSK4 (TLR1/2), lipopolisaccharide (TLR4), CpG ODNs (TLR9), L18-MDP (NOD2) and TDB (Mincle), leads to the activation of NF-kB. Stimulation with RLR ligands, such as transfected poly(I:C) or 5’ppp-dsRNA, or the STING agonist, 2’3’-cGAMP, triggers the IRF pathway. J774-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin®. 1. Jin M. et al., 2011. Effects of chondroitin sulfate and its oligosaccharides on toll-like receptor-mediated IL-6 secretion by macrophage-like J774.1 cells. Biosci Biotechnol Biochem. 75(7):1283-9. 2. Kushida T. et al., 2011. Enhancement of Dectin-2 gene expression by lignin-carbohydrate complex from Lentinus edodes mycelia extract (LEM) in a mouse macrophage-like cell line. Anticancer Res. 31(4):1241-8. 3. Takeda Y. et al., 2007. Ternary complex consisting of DNA, polycation, and a natural polysaccharide of schizophyllan to induce cellular uptake by antigen presenting cells. Biomacromolecules. 8(4):1178-86. 4. Wilden H. et al., 2009. Expression of RIG-I, IRF3, IFN-beta and IRF7 determines resistance or susceptibility of cells to infection by Newcastle Disease Virus. Int J Oncol. 34(4):971-82.
ID编号:
Size/Quantity数量:1 vial
Biosafety Level生物安全级别: 1
Shipping Info运输方式: Dry Ice
Storage储存方式: Liquid Nitrogen
Species物种:
Gender性别:
Product Type细胞类型:
Images图片:
Complete Growth Medium完全培养基:
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:
Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net
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