JIM3 Cell Line人骨髓瘤细胞株-BioVector NTCC典型培养物保藏中心

Cell Line Name:

JIM3

Keywords:

Human myeloma, cancer

Cell Line Description:

NTCC^TMJIM3 cell line is a myeloma tumour line demonstrating typical plasma cell phenotype (CD38, PCA-1 8A and CD24 positive), deficient in DNA repair. JIM3 was derived from plasma myeloma cells derived from pleural fluid of advanced multiple myeloma patient, homoplastic with NTCC^TMJIM1. Used for in vitro study and comparison of genetic instability in myeloma tumour lines (homoplastic with JIM1) and the in vitro study of effects of DNA repair deficiency in myeloma tumour lines

Species:

Human

Tissue of Origin:

Blood

CellType:

Small spherical

Growth Mode:

Suspension

Subculture Routine:

Seed at 2-9 x 10⁵ cells/cm3 5% CO2; 37°C.

Culture Medium:

Dexter culture medium, consisting of Fischer's medium + 20% FCS +10-7 M hydrocortisone sodium succinate

References:

Hamilton MS, Barker HF, Ball J, Drew M, Abbot SD, Franklin IM. (1991) Normal and neoplastic human plasma cells express bcl-2 antigen. Leukemia.5(9):768-71 PMID: 1943229.

Additional Bibliography:

Velangi MR, Matheson EC, Morgan GJ, Jackson GH, Taylor PR, Hall AG, Irving JA. (2004) DNA mismatch repair pathway defects in the pathogenesis and evolution of myeloma. Carcinogenesis. 25(10):1795-803 PMID: 15142887