6xHis-MBP-BrCas12b plasmid vector原核基因编辑质粒BrCas12b表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

6xHis-MBP-BrCas12b plasmid vector原核基因编辑质粒BrCas12b表达载体

Expresses recombinant BrCas12b

BACKBONE
Vector backbone pET
Backbone manufacturerScott Gradia Lab
Backbone size w/o insert (bp)6462
Total vector size (bp)9673
Vector typeBacterial Expression, CRISPR, Synthetic Biology
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberUnknown
GENE/INSERT
Gene/Insert nameBrCas12b
SpeciesBrevibacillus sp. SYP-B805
Insert Size (bp)3270
GenBank IDWP_165214399.1
PromoterT7
Tag / Fusion Protein
6xHis, T7, Thrombin site (N terminal on insert)
CLONING INFORMATION
Cloning methodGibson Cloning
5′ sequencing primertaatacgactcactatagg
3′ sequencing primerGCTAGTTATTGCTCAGCGG

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net