Parabacteroides merdae strain粪副拟杆菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Parabacteroides merdae strain粪副拟杆菌株

Type strain. This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.
Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI T4-1 [CIP 104202T, JCM 9497]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Feces
Geographical isolation
United States; Texas
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Characteristics
Comments
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions.
Under anaerobic conditions, withdraw 0.5 to 1.0 mL of #1490 broth from a single test tube (5 to 6 mL) with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #1490 agar slant and/or a pre-reduced #260 plate. An aerobic blood plate may be streaked to check for purity.
Incubate the tubes and plate under anaerobic conditions at 37°C for 2 to 6 days. Incubate the purity plate aerobically at 37°C.


ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

Use of an anaerobic gas chamber.
Placement of test tubes under a gassing cannula system hooked to anaerobic gas.


Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in anaerobic chamber.
Loose screw caps on test tubes in an activated anaerobic gas pack jar.
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

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