Exontrap vector pET01外显子捕获载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

Exontrap vector pET01外显子捕获载体质粒

Features of Exontrap vector. The intrinsic splicing function of the Exontrap vector is realized by a given exon/intron/exon structure within the vector. The vector-encoded intron sequence contains a multiple cloning site for inserting the genomic fragment of interest. Upstream of the first vector-encoded exon, a truncated part of the eukaryotic phosphatase gene is included. Downstream of the second vector-encoded exon is a polyadenylation signal localized. The transcription of the phosphatase-exon-intronexon area is under control of the strong „Long Terminal Repeat“ (LTR) promoter of the Rous Sarcoma Virus (RSV). Any genomic DNA that has been inserted into the MCS before, is now included in the synthesized pre-mRNA. After processing, a poly(A)+ mRNA is generated. This contains (5’ to 3’) the non-functional phosphatase gene, the first vector-encoded exon, all exons of the cloned genomic fragment, the second vector-encoded exon, and a polyA tail. All intron sequences are spliced off. For cloning in E. coli, the Exontrap vector contains a bacterial origin of replication, belonging to the ColE1 incompatibility group, and the b-lactamase gene coding for ampicillin resistance. Propagation in eukaryotic cells expressing large T antigens is facilitated through the SV40 origin.

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