Fibrobacter succinogenes strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Fibrobacter succinogenes strain菌株

Type strain
Product category
Bacteria
Product type
Anaerobe
Strain designation
S85 [VPI 12249; L.A. Burkey S85]
Type strain
Yes
Isolation source
Bovine rumen
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Specific applications
Produces restriction endonuclease FsuI
Produces xylan endo-1,3-beta-xylosidase xylan hydrolase, xylanase
Produces trypsin-resistant xylanases
Biotechnology

Comments
Medium must be absolutely reduced. Rehydrate vial in 5 mL broth, and immediately dilute 1:5 to a second broth tube. Strain grows best in a stabbed slant or in broth which contains very little cryoprotective agent.
Cellobiose transport

Medium 1943: Fibrobacter medium
Temperature
37°C
Atmosphere
97% CO2, 3% H2
Handling procedure
Perform all steps under anaerobic conditions.
Open vial according to enclosed instructions.
Using a single tube of #1943 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer the entire rehydrated contents of the vial into 5 to 6 mL of #1943 broth and immediately dilute 1:5 to a second broth tube. Medium must be absolutely reduced. Strain grows best in a stabbed slant or in broth that contains very little cryoprotective agent. Inoculate a plate of a non-selective medium such as Tryptic Soy, Nutrient, or blood agar with 0.1 mL of the cell suspension.
Seal the tube with a rubber stopper or use Hungate tubes and incubate anaerobically at 37°C. Incubate the plate aerobically as a purity check.
After 3-4 days, growth should be evident as turbidity throughout the broth. Once growth has been established, the culture should be transferred to fresh broth every 48 hours. No growth should appear on the plate incubated aerobically.

ANAEROBIC CONDITIONS
·Tubes of media are placed under a gassing cannula system connected to a source of oxygen free gas.
·All transfers are performed while the test tubes are on the cannula system with a gentle stream of oxygen free gas flowing through the system.
·As the test tubes are removed from the cannula system each is sealed with butyl rubber stopper thus maintaining the anaerobic headspace.
·This strain typically is grown in the presence of 97% carbon dioxide-3% hydrogen as a gas mixture.
Handling notes
Anaerobe Systems Brucella blood agar may be used for solid medium.

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