MDO E.coli strain大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MDO E.coli strain大肠杆菌菌株

A procedure for the isolation of mutants affected in
components containing glycerol derived from phospholipids yielded two mutant strains that contain membrane-derived oligosaccharides (MDO) devoid of glycerol (Rotering, H., Fiedler, W., Rollinger, W., and
Braun, V. (1984) FEMS Microbiol. Lett. 22, 61-68).
MDO are found in the periplasmic space of Escherichia
coli and other Gram-negative bacteria, and they may
comprise up to 7% of the cells dry weight. The biosynthesis of MDO is osmoregulated (Kennedy, E. P. (1982)
Proc. Natl. Acad. Sci. U. S. A. 79, 1092-1096) and
linked to the metabolism of phospholipids (van Golde,
L. M. G., Schulman, H., and Kennedy, E. P. (1973)
Proc. Natl. Acad. Sci. U. S. A. 70, 1368-1372). This
leads to substitution of MDO with sn-l-phosphoglycerol and phosphoethanolamine (Kennedy, E. P., Rumley, M. K., Schulman, p., and van Golde, L. M. G.
(1976) J. Biol. Chem. 261, 4208-4213). MDO also
contain succinate in 0-ester linkage. We now report
that one mutant strain lacks phosphoglycerol transferase I activity and thus is unable to transfer sn-lphosphoglycerol residues from phosphatidylglycerol to
MDO. The mdoB gene affected in this mutant has been
located at 99.2 min on the E. coli chromosome. The
ethanolamine content of MDO isolated from the mutant
strain is elevated, whereas the number of succinate
residues is not affected. The only phenotype of mdoB
mutants we found is a dramatic reduction of the diglyceride content observed in dgk mdoB double mutants when the &glucoside arbutin is present in the
growth medium.

Strain
WR1 gpsA glpD R glpKphA fadE rel-1 fhuA XKL16 Hfr thi-1 rel-1 XRelevant markers Source and
reference
WF21-1 mdoB::TnlO mutant of WR1
WF16
DF214
WF214
TlOGP
FlOGP
RZ600
RZ60
WF60
PC0135
AN261
WF261
HfrR5
KL208
KL25
HfrH
W2252
AB1157
WF1157
JEF8
WFF8
GM18
WF18
c9
MC4100
MH225
mdoB::TnlO transductant of KL16
F- hisGl rpsLll5 A (eda-zwf)l5pgi-7::Mul
mdoB:TnlO transductant of DF214
F- ilv metE lacI rpsL mdoA1
mdoB:TnlO transductant of TlOGP
HfrH thi-1 rel41
dgk-6 derivative of RZ600
mdoB::TnlO transductant of RZ60
F- thi-1 purE55 lacy1 mal41 xyl-7 mtl-2gal-3strepAll7
F- leu proC lac trp fep402 fhuA? rpsL
mdoB:TnlO transductant of AN261
Hfr thi-1 lacy1 mtl-2 xyl-7 mal41 gal-3 X’ supE44?
Hfr relAl? XHfr supE42 XHfr rel41 thi
HfrC metB
thr ara leu proA lacy gal his arg xyl tsx mtl rpsL
mdoB:TnlO transductant of AB1157
Hfr thr-31 carB8 reMl metB1 X- spoTl
mdoB::TnlO transductant of JEF8
F- thr-1 ara-14 leuB5 proA2 lacy1 galK2 his-4 thyAl2
supE44? X’ T5’
rpsL31 xyl-5 metBl thi-1 mtl trmBl tsx-33 deoBl6
supE44
mdoB:TnlO transductant of GM18
HfrC phoR18 reM1 fhuA22 Tzrpit-10 spoT1
araD139 lacU169 rpsL rel4 thi flbB
like MC4100. but + (om~C’-lacZ+)10-25 ul(209)
MH513 like MC4100; but @ iom&”-lacZ+j16-23 ipi(209)
TL1105 trkA trkD Ioc179 @ (kdpA5’-lacZ+)
e Strain collection of this institute.
-’ (12, 13)
-a (12)
This study
D. Fraenkel(24) via CGSC
This study
This study
C. R. H. Raetz (15)
C. R. H. Raetz (15)
This study
-
-’ (7, 14)
-
0
This study
CGSC
CGSC
CGSC
J. Hirota
This study
CGSC
This study
-
- 0
-
-
This study
CGSC
M. J. Casabadan (16)
T. J. Silhavy (17)
T. J. Silhavy (17)
W. Epsteini18)

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