pRNAT-CMV3.1-Puro siRNA哺乳动物基因沉默载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRNAT-CMV3.1-Puro siRNA哺乳动物基因沉默载体质粒

The human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on the RNA polymerase II system, the CMV promoter drives higher-level constitutive expression of genes in a greater variety of mammalian cell lines than RNA polymerase III-based promoters, such as U6 and H1. A CMV promoter drives the expression of siRNA, and an SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Afl II sites. The siRNA sequence can be confirmed by DNA sequencing using reverse sequencing primer DA0012 (5’-TAGAAGGCACAGTCGAGG-3’). This vector also carries cGFP (coral GFP) for convenient tracking of transfection efficiency.


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