ATCC Medium: 602 E Medium for Anaerobes 培养基 BioVector NTCC质粒载体菌种细胞基因保藏中心

ATCC Medium: 602 E Medium for Anaerobes 培养基

ATCC Medium: 602 E Medium for Anaerobes Base MediumRumen fluid (see below)…………………………..300mlGlucose…………………………………………….0.5gMaltose…………………………………………….0.5gSoluble Starch ……………………………………..0.5gPeptone……………………………………………..0.5gYeast Extract……………………………………….0.5g(NH4)2SO4…………………………………………0.4 gResazurin Solution (see below)…………………….4.0mlSalts Solution (see below)………………………….500 mlL-Cysteine x H2O………………………………….0.5gDI Water……………………………………………200 mlMix all of the ingredients in an anaerobe jar. Bring jar to a gentle boil. Cool under 80:20gas for approximately 10-20 minutes until medium changes from pink to yellowish. Theflow of gas should cause gentle bubbling (sufficient to exclude air). Add 0.5 g L-cysteinex H2O. Adjust pH to 7.0 if required with 6N NaOH or 5N HCl. Dispense anaerobicallyand stopper with butyl rubber stoppers. Autoclave at 121ºC for 15-20 minutes.Rumen FluidFilter rumen contents, obtained from a cow fed on an alfalfa-hay concentrate ration,through two layers of cheesecloth to remove large particles. Store under CO2 in quartmilk bottles in the refrigerator. Much of the particulate matter settles. Use only thesupernatant.Resazurin SolutionDissolve 11mg resazurin in 44 ml of distilled water.Salts SolutionCaCl2 (anhydrous)………………………………0.2 gMgSO4…………………………………………..0.2 gK2HPO4…………………………………………1.0 gKH2PO4…………………………………………1.0 gNaHCO3…………………………………………10.0 gNaCl………………………………………………2.0 gMix CaCl2 and MgSO4 in 300 ml of distilled water until dissolved. Add 500 ml waterand add the remaining salts while swirling slowly. Add 200 ml of distilled water, mix

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