Human Liver Cancer Stable Cell Line Huh7, GFP/puro Overexpressed (CAR-STC-ZP51)稳转细胞株

Specifications
SpeciesHuman
Cell TypeHuman liver cancer cells
Overexpressed GeneGFP-puro
Size3x10^6 cell/ vial
Culture ConditionDMEM+10%FBS
Growth PatternAdherent culture
Cell Purity>95%
Cell Viability>90%
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
ShippingDry ice
Storage-80°C for short-term storage, liquid nitrogen for long-term storage
Handling NotesCell Recovery:

1. Put on a protective mask and antifreeze gloves, remove the cells from the liquid nitrogen tank, and quickly put them in a 37°C water bath to thaw.

2. After the cells are thawed, transfer the cells to the cell chamber through the transfer chamber.

3. In the biological safety cabinet, take out a 15mL sterile centrifuge tube, and use a 1mL pipette to add 4mL medium to the 15mL centrifuge tube.

4. Use a 75% medical disinfectant alcohol cotton ball to carefully wipe the outer surface of the cell cryopreservation tube that has been thawed in a 37°C water bath for surface disinfection. In the biological safety cabinet, unscrew the cell cryopreservation tube, then use a 1mL pipette to aspirate the cell suspension in the tube, add it dropwise to the 15mL centrifuge tube containing 4mL medium prepared above, and screw the lid of the 15mL centrifuge tube. Tightly, slowly turn upside down 4 times to mix the cell suspension.

5. Place the centrifuge tube containing the cell suspension in the centrifuge and set the centrifugation parameters as follows: Centrifuge at 200xg at room temperature (~25°C) for 5 minutes.

6. After centrifugation, gently transfer the centrifuge tube to the biological safety cabinet. At this time, white cell clusters can be seen at the bottom of the cytocentrifuge tube. Use an electric aspirator to aspirate the medium supernatant (the cell cluster is relatively loose, be careful not to touch the cell cluster at the bottom).

7. Use a 1mL pipette to add 2mL of complete medium, gently pipette 5 times to resuspend the cell pellet, count, and inoculate.

8. Place the cells in an incubator for overnight culture.
WarningsAvoid multiple freeze/thaw cycles
Research Use OnlyOur recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
TumorgenicityPositive (In vitro/vivo transformation assay)
OncogenicityPositive (In vitro soft agarose assay and life-time studies )
Sterility TestingBioVector NTCC Inc. provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity TestingIdentity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety TestingA broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability TestingWe perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Overexpressed GeneGFP-puro