GFP+-Stable Cell Line-Raji (SC-LX001)稳转细胞株

Raji cells which are CD19+ are generated using lentiviral GFP transduction (CD19+eGFP cells). These cells can be used as target cells for CAR-T with CD19-CAR-T cells. The cells stably express GFP and are about 80% positive for GFP expression.

Specifications
Cell BackgroundRaji cell is established in 1963 from Burkitt's lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA.
SpeciesHuman
Cell TypeRaji
Overexpressed GeneGFP
Culture ConditionSuspension
Growth PatternExponential growth phase
FormulationContaining ≥ 1 X 10*6 / vial lyophilized cells
Cell Purity>95%
Cell Viability>90%
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
ShippingDry ice
StorageLyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling NotesFrozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
WarningsAvoid multiple freeze/thaw cycles
Research Use OnlyOur stable cell line are for research use only, not for diagnostic or therapeutic use.
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
TumorgenicityPositive (In vitro/vivo transformation assay)
OncogenicityPositive (In vitro soft agarose assay and life-time studies)
Sterility TestingBioVector NTCC Inc. provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity TestingIdentity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety TestingA broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability TestingWe perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
ApplicationThe cells can be used for cytotoxicity assays or IFN-gamma release assay similarly to Raji cells.
Overexpressed GeneGFP
CAR Design
IntroductionThe Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt's lymphoma of the left maxilla of an 11-year-old Black male. These cells are EBNA positive and are partially resistant to poliovirus and vesicular stomatitis viruses. The GFP Stable Cell Line-Raji constitutively expresses GFP.
Target SpeciesHuman
GeneID118220082