GFP+-Stable Cell Line-K562 (SC-LX003)稳转细胞株

K562 cells which are CD19-negative are generated using lentiviral GFP transduction. These cells can be used as a negative control target cells for CAR-T with CD19-CAR-T cells.

Specifications
Cell BackgroundK562 cell line is a cell line from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.
SpeciesHuman
Cell TypeK562
Overexpressed GeneGFP
Culture ConditionSuspension
Growth PatternExponential growth phase
FormulationContaining ≥ 1 X 10*6 / vial lyophilized cells
Cell Purity>95%
Cell Viability>90%
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
ShippingDry ice
StorageLyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling NotesFrozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
WarningsAvoid multiple freeze/thaw cycles
Research Use OnlyOur stable cell line are for research use only, not for diagnostic or therapeutic use.
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
TumorgenicityPositive (In vitro/vivo transformation assay)
OncogenicityPositive (In vitro soft agarose assay and life-time studies)
Sterility TestingBioVector NTCC Inc. provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity TestingIdentity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety TestingA broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability TestingWe perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
ApplicationThese cells are CD19-negative and can be used as a negative control for CD19-CAR-T cells in cytotoxicity assays as shown with K562 cells.
Overexpressed GeneGFP
CAR Design
IntroductionK562 cell line is a cell line from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.GFP+-Stable Cell Line-K562 constitutively expresses GFP.
Target SpeciesHuman
GeneID118220082