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CD37+-GFP+-Stable Cell Line-CHO (SC-LX014)稳转细胞株

  • 价  格:¥989865
  • 货  号:CD37+-GFP+-Stable Cell Line-CHO (SC-LX014)
  • 产  地:北京
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BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:400-800-2947 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

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This CHO cell line stably overexpresses CD37 antigen and GFP. This cell line is convenient for assays with CAR-T cells to measure specific cytotoxicity targeting CD37 antigen, which is overexpressed in cancer cells.

Specifications
Cell BackgroundCHO cells are Chinese hamster ovary cells-are epithelial cells derived from the ovary of the Chinese hamster, often used in biological and medical research in the production of therapeutic proteins. This cell line have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics.
SpeciesHamster
Cell TypeCHO
Overexpressed GeneCD37&GFP
Culture Conditionadherent
Growth PatternExponential growth phase
FormulationContaining ≥ 1 X 10*6 / vial lyophilized cells
Cell Purity>95%
Cell Viability>90%
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
ShippingDry ice
StorageLyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling NotesFrozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
WarningsAvoid multiple freeze/thaw cycles
Research Use OnlyOur stable cell line are for research use only, not for diagnostic or therapeutic use.
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
TumorgenicityPositive (In vitro/vivo transformation assay)
OncogenicityPositive (In vitro soft agarose assay and life-time studies)
Sterility TestingBioVector NTCC Inc. provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity TestingIdentity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety TestingA broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability TestingWe perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
ApplicationThe CHO-CD37 cells are available for functional assays with CD37-CAR-T cells, CD37-bi-specific antibodies, can be used for in vivo xenograft studies, and more.
Overexpressed GeneCD37&GFP
CAR Design
IntroductionCHO cells are Chinese hamster ovary cells-are epithelial cells derived from the ovary of the Chinese hamster, often used in biological and medical research in the production of therapeutic proteins. This cell line have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics.CD37+-GFP+-Stable Cell Line-CHO constitutively expresses CD37+-GFP.
Target SpeciesHuman
GeneID951
118220082

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