Helicobacter pylori strain幽门螺杆菌菌株700684 BioVector NTCC质粒载体菌种细胞基因保藏中心

Helicobacter pylori strain幽门螺杆菌菌株700684

Product category
Bacteria
Strain designation
UA 1182
Type strain
No
Isolation source
Gastric biopsy; isolated June 1993 by N. Chang at University of Alberta
Geographical isolation
Canada; Alberta
Applications
Enteric disease research
Infectious disease research

Specific applications
Enteric Research

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Microaerophilic
Handling procedure
Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Aseptically transfer the entire contents to a 5-6 mL tube of #18 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Or, to obtain a biphasic culture, add several drops of the primary broth tube to a #260 agar slant. Best practice is to incubate these slants at an angle.
Incubate at 37°C under microaerophilic conditions for 3 days. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes and slants should be incubated with caps loosened.
Handling notes
This is a slow growing organism that requires moist conditions for best growth.  Growth at the broth/agar interface of the biphasic slant should occur within three days, but little turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy.  Motility is usually observed only in young cultures. The presence of spheroid cells indicates that viability is being lost either due to age or too much exposure to oxygen.

Growth on agar takes longer than with the biphasic culture.  Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods.  Viability also decreases with repeated subculturing.  The cells do not Gram stain well using traditional procedures.  To obtain the best results, use a basic fuchsin counterstain in place of the safranin.

Once good growth is obtained, transfer or freeze the culture.  Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or "ultra-low temperature" freezer is recommended.

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