Helicobacter pylori 700392 strain幽门螺杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Helicobacter pylori 700392 strain幽门螺杆菌菌株

Product category
Bacteria
Strain designation
26695 [KE26695]
Type strain
No
Genome sequenced strain
Yes
Isolation source
Stomach of patient with gastritis
Geographical isolation
United Kingdom
Applications
Bioinformatics
Enteric disease research
Infectious disease research

Specific applications
Enteric Research
Genome sequencing strain

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Microaerophilic: 3-5% O2, 10% CO2
Handling procedure
This organism is shipped frozen in dry ice.  Just prior to use, thaw vial in water at approximately 37°C.  When thawed, a drop of the suspension may be used to do an immediate wet mount to observe the unique morphology of this organism and verify its viability by checking for motility.
Aseptically transfer the thawed suspension into a fresh #18 broth (3-5 mL).  Mix well.  This suspension can now be used to inoculate agar slant(s), plate(s), or the preferred biphasic culture.  Two #260 plates should be inoculated, one for microaerophilic growth and the second for aerobic growth.  No growth should occur on the plate incubated aerobically.
To obtain a biphasic culture, add 0.6 mL of the suspension to a #260 slant.  The resulting pool at the bottom of the slant is where the best, most rapid growth will occur.
Incubate at 37°C under microaerophilic conditions using an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method, to obtain microaerophilic conditions.  Incubate tubes with cap loose.
Within 2-3 days, good growth should be obtained in the broth pool at the bottom of the slant and on the microaerophilic plate.  Further subcultures can be made using the broth pool as the inoculum source.  Subcultures to biphasic cultures will require only 24 to 48 hours of incubation for good growth.

Handling notes
Growth at the broth/agar interface of the biphasic slant should occur within 3 days, but only light turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy.


Growth on agar takes longer than the biphasic culture. The cells do not Gram stain well using traditional procedures. For best results, use a basic fuchsin counterstain in place of the safranin.



Once good growth in obtained, transfer or freeze the culture. Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or “ultra-low temperature” freezer is recommended.



Purified genomic DNA of this strain is available

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