pNLAD8 plasmid vector HIV-1传染性克隆质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNLAD8 plasmid vector质粒载体

pNLAD8 contains a full-length replication-competent, infectious human immunodeficiency virus 1 (HIV-1), subtype B, strain NL4-3 AD8 chimeric molecular clone. The virus produced by this molecular clone utilizes the co-receptor CCR5.

The source of this chimeric molecular clone is HIV-1 strains AD8-1 and NL4-3. This molecular clone contains the genetic backbone of pNL4-3, with AD8-1 env. This CCR5-utilizing derivative of pNL4-3 was constructed by removing a portion of pNL4-3 (nucleotides 6348 to 8051) and replacing it with a 1.7-kb fragment from pAD8.1. This 1.7 kp fragment in pAD8-1 corrensponds to the region between the Kpnl site in the gp120 coding region, and the Bsml site in gp41. The resulting infectious molecular clone is able to utilize the co-receptor, CCR5, while the original pNL4-3 utilized the CXCR4.

The complete genome sequences for pNL4-3 and AD8 are available (GenBank: AF324493 and AF004394).

Alternate names include: pNL(AD8), HIV-1 AD8 Macrophage-Tropic R5.

ARP-11346 contains the ampicillin resistance gene. The size of this construct is 14,828 base pairs including the insert. The size of the insert is 1,706 bp.

Note: pNLAD8 can be propagated in STBL2™ cells and grown at 37°C. Larger plasmids may benefit from growth at 30°C. This construct may also be grown in other competent cells.

Each vial of pNLAD8 contains approximately 5 µg of dried purified DNA stabilized in DNAstable®PLUS.

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