RAW264.7小鼠单核巨噬细胞白血病细胞 - BioVector NTCC典型培养物保藏中心
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RAW264.7 Cells. 小鼠单核巨噬细胞白血病细胞 【种属】： 小鼠 【组织】： 血液
RAW264.7 Cells. 小鼠单核巨噬细胞白血病细胞
【种属】： 小鼠 【组织】： 血液
General Cell Collection: RAW 264.7
| Catalogue No.: | 91062702 |
|---|---|
| Cell Line Name: | RAW 264.7 |
| Keywords: | Mouse monocyte macrophage |
| Cell Line Description: | Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abelson Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS. |
| Species: | Mouse |
| Tissue: | Blood |
| Morphology: | Macrophage |
| Growth Mode: | Semi-adherent |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO2; 37°C. Use cell scrapers to remove attached cells. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic. |
| Culture Medium: | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Karyotype: | Not specified |
| Products: | Lysozyme |
| Receptors: | Immunoglobulin, complement |
| Country: | USA |
| References: | J Immunol 1977;119:950; Cell 1978;15:261 |
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