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H1869 cell line细胞株
NCI-H1869 [H1869] cell line
Cat No.: NTCC-CRL5900
Organism
Homo sapiens, human
Tissue
lung
Cell Type
squamous cell
Product Format frozen
Culture Properties adherent, adherent
Biosafety Level 1.
Disease
stage 4, non-small cell lung cancer
Age
58 years
Gender
male
Ethnicity
Caucasian
Derivation
The line was established in March 1988 from pleural effusion, a metastatic site.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker. 50 pack years.
Complete Growth
Medium
ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is DMEM: F12 Medium. To make the complete growth
medium, add the following components to the base medium:
1. 0.02 mg/ml insulin
2. 0.01 mg/ml transferrin
3. 25 nM sodium selenite
4. 50 nM Hydrocortisone
5. 1 ng/ml Epidermal Growth Factor (do not filter)
6. 0.01 mM ethanolamine
7. 0.01 mM phosphorylethanolamine
8. 100 pM triiodothyronine
9. 0.5% (w/v) bovine serum albumin
10. 10 mM HEPES
11. 0.5 mM sodium pyruvate
12. 2mM L-glutamine(for final conc. of 4.5 mM)
13. 10% FBS
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase
amount of dissociation medium for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of
serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope
until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells
to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:4 to 1:6
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines
consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian
Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation RPMI 1640 medium 85%; fetal bovine serum, 10%; DMSO, 5%.
Culture Conditions Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,9
TH01: 6
TPOX: 8,9
vWA: 16
Year of Origin
1988
References
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24:
1996
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