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H157 cell line人肺鳞状细胞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥48965
  • 货  号:H157
  • 产  地:北京
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H157 cell line人肺鳞状细胞癌细胞株

Antigen/Gene or Protein Targets Human oral squamous cell carcinoma
Tissue Buccal mucosa
Disease Keywords Cancer
Model Tumour line

Relevance Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84. STNMP stage II, well differentiated, node positive tumour. Mutant p53, codon 306 exon 8, G to A; wild type K-, N- and Ha-ras. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic), but form epidermoid cysts subcutaneously
Research Area Cancer

Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:8 to 1:10 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm². DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.

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