INS-1E cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

INS-1E cell line细胞株

Disease:
Insulinoma (Isolated clonal INS-1E cells from the parental INS-1 cells)

Origin:
Pancreatic Islets

Species:
Rattus Norvegicus

Tissue:
Pancreas

Morphology:
β cells

Properties:
Adherent

Medium:
BioVector-Optimized RPMI-1640 Medium + 10% FBS + 0.05 mM 2-mercaptoethanol

Subculture:
1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C

Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:
I

Sterility:
Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:
HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

Insulin Responsiveness:
Respond to glucose within the physiological range. Relatively high insulin content.

Image:


References for INS-1E cells:
1. Janjic D, Maechler P, et al. (1999). Free radical modulation of insulin release in INS-1 cells exposed to alloxan. Biochem. Pharmacol. 57, 639-648.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net