Stable Competent E. coli (High Efficiency)慢病毒质粒防重组克隆菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

Stable Competent E. coli (High Efficiency)慢病毒质粒防重组克隆菌株/感受态细胞

Stable Competent E. coli (High Efficiency)
Supplied with outgrowth medium optimized for NEB 10-beta & NEB Stable Competent E.coli ; please do not use SOC outgrowth medium.

Chemically competent E. coli cells suitable for high efficiency transformation.

Ideal for isolation of plasmid clones containing repeat elements and unstable inserts
Useful for isolating and propagating retroviral/lentiviral clones
Carries the endA mutation (in contrast to Stbl3) so that isolated plasmids are free of Endonuclease I
No dry ice surcharge on competent cell shipments
Outgrowth medium included
Free of animal products

Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.

Highlights
Transformation efficiency:
1-3 x 109 cfu/µg pUC19 DNA
Recommended host strain for cloning genes into retroviral/ lentiviral vectors
Recommended for cloning of direct repeats and inverted repeat sequences
Reduced recombination of cloned DNA (recA1)
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening with vectors capable of alpha-complementation
Genotype
F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR)/ ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14-  Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC)

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947

www.biovector.net
Website网址: http://www.biovector.net