Venus-krasCt plasmid vector哺乳动物细胞膜定位荧光标记质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Venus-krasCt plasmid vector哺乳动物细胞膜定位荧光标记质粒载体

Membrane marker
The Venus KRasCT fragment was made as a gBlock with HindIII and XbaI, cut and cloned into pcDNA3.1

Vector backbonepcDNA3.1
Backbone manufacturerinvitrogen
Backbone size w/o insert (bp)5428
Total vector size (bp)5926
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameVenus KRasCT
SpeciesSynthetic
Insert Size (bp)798
PromoterCM
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteHindIII (not destroyed)
3′ cloning siteXbaI (not destroyed)
5′ sequencing primerT7
3′ sequencing primerBGH

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net