2E8 cell line小鼠骨髓B淋巴细胞株TIB-239 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99865
- 货 号：2E8 cell
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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2E8 cell line小鼠骨髓B淋巴细胞株TIB-239
Product categoryAnimal cellsOrganismMus musculus, mouseClassificationEukaryota, Animalia, Metazoa, Chordata, Vertebrata, TetrapodCell typeB lymphocyteMorphologylymphoblastTissueBone; MarrowApplications3D cell cultureImmunologySpecific applicationsThe 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Growth propertiesSuspensionAgejuvenileStrainBALB/c.xidAntigen expressionH-2 d; Ia d; BP-1Genes expressedimmunoglobulin (surface IgM)Expression markersInterleukin 7 (IL-7)IsotypeIgMCommentsThe 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Complete mediumIscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serumTemperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 ml complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product.Subculturing procedureEvery 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 105 viable cells/mL by adding fresh medium to the culture. Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853). The cells do not grow as well when human IL-7 is substituted for mouse IL-7.Medium Renewal: Every 2 to 4 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Product categoryAnimal cellsOrganismMus musculus, mouseClassificationEukaryota, Animalia, Metazoa, Chordata, Vertebrata, TetrapodCell typeB lymphocyteMorphologylymphoblastTissueBone; MarrowApplications3D cell cultureImmunologySpecific applicationsThe 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Growth propertiesSuspensionAgejuvenileStrainBALB/c.xidAntigen expressionH-2 d; Ia d; BP-1Genes expressedimmunoglobulin (surface IgM)Expression markersInterleukin 7 (IL-7)IsotypeIgMCommentsThe 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Complete mediumIscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serumTemperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 ml complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product.Subculturing procedureEvery 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 105 viable cells/mL by adding fresh medium to the culture. Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853). The cells do not grow as well when human IL-7 is substituted for mouse IL-7.Medium Renewal: Every 2 to 4 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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