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gWIZ-EGFP,PVAX1-EGFP DNA疫苗载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥989865
  • 货  号:gWIZ-EGFP,PVAX1-EGFP
  • 产  地:北京
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gWIZ-EGFP,PVAX1-EGFP DNA疫苗载体质粒

DNA vaccine vectors. (a,b) 1st; (c) 2nd; and (d) 3rd; generation DNA Vaccine vectors; (e) 2nd and 3rd generation vectors increase in vivo expression compared to first generation vector gWIZ. 5 µg muSEAP vectors delivered intramuscularly with EP to mice on day 0, serum muSEAP assayed on indicated days. 3rd generation vector NTC9385R has significantly higher expression than gWIZ or 2nd generation vector NTC8385 (p-value = 0.05; Mann-Whitney rank-sum test); (f) 3rd generation vectors dramatically increase in vivo expression, compared to 2nd generation. 50 µg muSEAP vectors in 50 µL saline delivered intradermally to mice with EP on day 0, muSEAP assayed on indicated days. 3rd generation vector NTC9385R has significantly higher expression than 2nd generation vector NTC8685 (p-value = 0.05; Mann-Whitney rank-sum test). NTC8685 is a 2nd generation vector similar to NTC8385. The NTC8385 1,518 basepair (bp) bacterial region (spacer region) is reduced to 855 bp in NTC8385-min and 454 bp in NTC9385R. This compares to 2,678 bp for gWIZ, and 1,970 bp for pVAX1.

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