MM127 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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- 货 号:BioVector-CBA-1344
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
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地址:北京
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Description: MM127 is an adherent melanoma cell line; it was established in 1970 from a male patient. It was derived from a subcutaneous metastatic melanoma. Description Key Words: Human melanoma male Also Known As: No other names Organism: Human (Homo sapiens) Strain: N/A Tissue: Skin Growth Properties: Adherent Morphology: Epithelial Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 10% Foetal Calf Serum Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Subculturing Procedure: Medium Renewal: 2-3 times per week. Subcultivation Ratio: 1:8, Seeding density 0.6 x104cells/cm2. Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. Culture Conditions: Incubate the culture at 37°C with 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. Additional Information: Mutations:- Homozygous deletion CDKN2A- G13R mutation NRas Medium:Growth Condition:BioVector NTCC Inc.www.biovector.net
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