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SF10602 cell line细胞株
clones of the astrocytoma SF10602 PDC GNS and the oligodendroglioma SF10417 PDC GNS by sparsely seeding 500 cells into laminin-coated 15 cm tissue culture dishes. Colonies formed from single cells were isolated with cloning cylinders and Accutase (Innovative Cell Technologies). Seventeen clones were derived from SF10602 PDC GNS and 31 from SF10417 PDC GNS. Of the 48 clones, which were all IDH1-mutant by Sanger sequencing, we selected 5 SF10602 PDC GNS and 4 SF10417 PDC GNS clones for exome sequencing.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
clones of the astrocytoma SF10602 PDC GNS and the oligodendroglioma SF10417 PDC GNS by sparsely seeding 500 cells into laminin-coated 15 cm tissue culture dishes. Colonies formed from single cells were isolated with cloning cylinders and Accutase (Innovative Cell Technologies). Seventeen clones were derived from SF10602 PDC GNS and 31 from SF10417 PDC GNS. Of the 48 clones, which were all IDH1-mutant by Sanger sequencing, we selected 5 SF10602 PDC GNS and 4 SF10417 PDC GNS clones for exome sequencing.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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