pADL-10b plasmid vector噬菌体展示载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：pADL-10b
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pADL-10b plasmid vector噬菌体展示载体质粒
The pADL-10b phagemid is a type 3+3 phage display vector with a cloning site for display on the N-terminal side of the full-length gene III protein. Secretion in the periplasm of the fusion protein is driven by the PelB leader peptide.The pADL-2x phagemid vector series offers optimal characteristics for phage display with optimized expression of thefusion protein for strong display, suppressible amber codon to direct the fusion either as a gene III fusion or as a secretedfree entity, cloning site amenable to multiple cloning strategies and varied linker and display options. The fusion protein isunder the control of the lac promoter, allowing metabolic repression by glucose and induction by IPTG. A copy of thelambda t1 terminator located downstream gene III prevents leakiness of the transcription during induction, in particularpreventing excessive expression of the beta-lactamase and rapid consumption of ampicillin.The vector contains two origins of replication, the f1 origin, which packages the single-stranded phagemid DNA into nascentvirions, and the pMB1 origin of replication derived from pBR322, which results in a high-copy-number phagemid. The pMB1sequence lacks the rop gene and carries a point mutation in the RNAII transcript (G 2975 in pBR322 to T 1304 on the reversecomplement strand responsible for a temperature-sensitive very high copy number phenotype (Lin-Chao 1992).
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The pADL-10b phagemid is a type 3+3 phage display vector with a cloning site for display on the N-terminal side of the full-length gene III protein. Secretion in the periplasm of the fusion protein is driven by the PelB leader peptide.The pADL-2x phagemid vector series offers optimal characteristics for phage display with optimized expression of thefusion protein for strong display, suppressible amber codon to direct the fusion either as a gene III fusion or as a secretedfree entity, cloning site amenable to multiple cloning strategies and varied linker and display options. The fusion protein isunder the control of the lac promoter, allowing metabolic repression by glucose and induction by IPTG. A copy of thelambda t1 terminator located downstream gene III prevents leakiness of the transcription during induction, in particularpreventing excessive expression of the beta-lactamase and rapid consumption of ampicillin.The vector contains two origins of replication, the f1 origin, which packages the single-stranded phagemid DNA into nascentvirions, and the pMB1 origin of replication derived from pBR322, which results in a high-copy-number phagemid. The pMB1sequence lacks the rop gene and carries a point mutation in the RNAII transcript (G 2975 in pBR322 to T 1304 on the reversecomplement strand responsible for a temperature-sensitive very high copy number phenotype (Lin-Chao 1992).
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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