pAAV-flex-taCasp3-TEVp plasmid vector质粒载体,腺相关病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAAV-flex-taCasp3-TEVp plasmid vector质粒载体,腺相关病毒

The taCasp3-T2A-TEVp transgene was generated by overlapping PCR of plasmids harboring taCasp3 and TEVp (Gray et al., 2010; Cell 142, 637-646 PMID: 20723762). This transgene was inserted in reverse orientation into the plasmid pAAV-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA such that it replaced hChR2(H134R)-EYFP

Vector backbonepAAV-MCS
Backbone manufacturerStratagene
Backbone size w/o insert (bp)5347
Total vector size (bp)7225
Vector typeAAV ; Adeno Associated Viral Vector
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature30°C
Growth Strain(s)Stbl2
Growth instructionsFor liquid cultures grow in LB:2XYT (1:1) overnight at 30 C.
Copy numberLow Copy
GENE/INSERT 1
Gene/Insert nameCaspase 3
Alt nameCasp3
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)847
MutationLinker replaced with a TEV protease cleavage site
Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
PromoterEF1a
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodUnknown
5′ sequencing primerAAA GCA GCG TAT CCA C
3′ sequencing primerAAT CAT GTC CCT GCC GTC GAT C
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert nameTobacco Etch Virus protease
Alt nameTEV protease
Insert Size (bp)705
PromoterEF1a
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodUnknown
5′ sequencing primerTTC TCA AGC CTC AGA CAG TGG
3′ sequencing primerAGA GGG GAT CGT TGT AGA AGT C

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