出品公司: | Clontech |
---|---|
载体名称: | pRetroX-IRES-DsRed Express, pRetroX-IRES-DsRedExpress |
质粒类型: | 哺乳动物细胞双顺反子逆病毒载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5934bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | IRES-R: CCTCACATTGCCAAAAGACG |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | -- |
备注: | 载体表达DsRed荧光蛋白作为筛选标志,用来构建稳表达细胞系。 |
产品目录号: | 632521 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
pRetroX-IRES-DsRed Express逆病毒载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorpRetroX-IRES-DsRed Express
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRetroX-IRES-DsRedExpress 5934 bp DNA circular 10-NOV-2009 COMMENT Created by Clontech Laboratories Inc. COMMENT Cat. No. 632521 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers LTR 1..592 /label=5'\MMLV\LTR misc_feature 662..1012 /label=Packaging\signal intron 1075..1308 /label=Intron\containing\splice\acceptor\site LTR 2699..3290 /label=3'\MMLV\LTR rep_origin 4143..4262 /label=pUC\origin CDS complement(4470..5327) /label=Amp(R) misc_feature 1392..1966 /label=IRES misc_feature 1967..2644 /label=DsRedExpress misc_feature 1361..1390 /label=MCS misc_feature 650..655 /label=Splice\donor\site BASE COUNT 1402 a 1571 c 1557 g 1404 t ORIGIN 1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag 121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgggctcaat 421 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg 481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggcct cgctgttcct 541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg 661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct 721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga 781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg 841 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag 901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg 961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg 1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatacg cgtgtcgagc 1081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 1141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 1201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 1261 ttttttcttc catttcagcc gtcgtgaaaa ctacccctaa aagccaaaaa aatcgaattc 1321 ccaaacttaa gcttggtacc gagctcggat ctcacgtggg cccgcggccg cagatctatc 1381 gatggatccg tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 1441 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 1501 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 1561 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 1621 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 1681 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 1741 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag 1801 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 1861 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc 1921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg cctcctccga 1981 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca 2041 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 2101 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 2161 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct 2221 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 2281 gaccgtgacc caggactcct ccctgcagga cggctccttc atctacaagg tgaagttcat 2341 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg gctgggaggc 2401 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct 2461 gaagctgaag gacggcggcc actacctggt ggagttcaag tctatctata tggccaagaa 2521 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa 2581 cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc acctgttcct 2641 gtagaatgct cgaggataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg 2701 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg 2761 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct 2821 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 2881 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc 2941 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag 3001 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa 3061 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa 3121 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta 3181 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg 3241 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt gaattcgtaa 3301 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 3361 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 3421 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 3481 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 3541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 3601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 3661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 3721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 3781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 3841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 3901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 3961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4141 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 4261 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 4321 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 4381 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 4441 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 4501 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 4561 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 4621 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 4681 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 4741 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 4801 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 4861 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 4921 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 4981 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5041 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 5101 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 5161 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 5221 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 5281 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 5341 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 5401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 5461 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 5521 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 5581 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 5641 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 5701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 5761 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 5821 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 5881 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tatg //
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