pMarVF1 plasmid vector费氏弧菌自杀质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMarVF1 plasmid vector费氏弧菌自杀质粒载体

Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114
Vector backbone：pSAM
Backbone manufacturerJeffrey I. Gordon, PMID: 19748469
Backbone size w/o insert (bp)3608
Total vector size (bp)4552
Modifications to backbonenrdR promoter was added using BamHI/NdeI sites to drive expression of himar1C9 transposase
Vector typeSuicide vector in V. fischeri ES114
Selectable markersErythromycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)S17-1 λpir
Copy numberUnknown
GENE/INSERT
Gene/Insert nameV. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
Insert Size (bp)944
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteXhoI (not destroyed)
3′ cloning siteXbaI (not destroyed)

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net