DM-3 cell line细胞株DM3 BioVector NTCC质粒载体菌种细胞基因保藏中心

DM-3 cell line细胞株DM3

Cell line:
DM-3
DSMZ no.:
ACC 595
Species:
human (Homo sapiens)
Cell type:
sarcomatoid mesothelioma
Origin:
established from a pleural biopsy of a 62-year-old man with malignant mesothelioma
Reference(s):
14850
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
adherent, sarcomatoid cells growing in monolayers; image; image ; image
Medium:
80% Dulbecco's MEM or NCTC-109 + 20% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:4 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109)
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 6-8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by species PCR
Cytogenetics:
human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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