RS-5 cell line细胞株RS5 BioVector NTCC质粒载体菌种细胞基因保藏中心

RS-5 cell line细胞株RS5

Cell line:
RS-5
DSMZ no.:
ACC 604
Species:
human (Homo sapiens)
Cell type:
sarcomatoid mesothelioma
Origin:
established in 2003 from the untreated malignant mesothelioma of the pleura of a 78-year-old man with asbestos cancer
Reference(s):
14850
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
adherent, sarcomatoid-like cells growing in monolayers; image; image; image
Medium:
90% NCTC-109 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow slowly for about one week after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3 days
Harvest:
maximum density of ca. 3 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded near-diploid karyotype with 5% polyploidy; 46(39-48)<2n>XY; although no consistent abnormality was detected, most metaphases showed random chromosome loss
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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