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Hela-CD4 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99865
  • 货  号:Hela-CD4
  • 产  地:北京
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Hela-CD4 cell line细胞株

HeLa CD4+
Comment A subline of the HeLa cells. Expressing human CD4 and Neomycin-resistant gene.
Basic information
Originator Bruce Chesebro
Year of deposit 2006
Original cell HeLa
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene human CD4 gene, neomycin resistance gene
History NIH AIDS Research & Reference Reagent Program
Lifespan infinite
Morphology epithelial-like


Medium
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK

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