pSLCAR-CD19-CD3z慢病毒CAR-T表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSLCAR-CD19-CD3z慢病毒CAR-T表达质粒载体

Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameFMC63-3z-P2A-EGFP
Alt nameCD19(FMC63)-CAR, no costim., CD3z stim domains, P2A-GFP marker
Alt nameContains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, CD3z-ITD
SpeciesH. sapiens (human)
Entrez GeneCD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ)
Entrez GeneCD28 (a.k.a. Tp44)
PromoterEF1a-short
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBpiI (destroyed during cloning)
3′ cloning siteBpiI (destroyed during cloning)
5′ sequencing primerCGGGTTTGCCGCCA
3′ sequencing primercaggagccTGCTCCTCTTACTcc

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net