U2OS-LacO-I-SceI-TetO Cell Line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

U2OS-LacO-I-SceI-TetO Cell Line细胞株

U2OS bone osteocarcinoma cell line integrated with I-Scel endonuclease site in-between a lac operator (lacO) sequence and a tet operator (TetO) sequence. This allows for a Lac-repressor/operator-tethering system designed to probe the role of chromatin condensation in DNA damage response (DDR).

Highlights:

Consists of 10 kb tandem arays of the Lac operator (lacO) sequence adjacent to an I-SceI endonuclease site stably integrated into human U2OS cells at two different chromosomal locationsDefined chromatin domains can be generated by virtue of tethering fusion proteins between chromatin modifiers and the Lac repressor (LacR) protein, which binds with high affinity to the lacO regions
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. U2OS is an established human epithelial cell line isolated back in the 1960s from a 15 year old female suffering from bone cancer or osteosarcoma. The cells came from a moderately differentiated sarcoma on the tibia and allow for fast growth and have been shown to have high transfection efficiencies.
Product Type: Cell Line
Name: U2OS
Cell Type: Bone Osteosarcoma
Organism: Human
Morphology: Epithelial
Biosafety Level: BSL1
Growth Conditions: MEM, 10% FBS, Penn Strep, Glutamine, 200ug/mL hygromycin
Cryopreservation: FBS and 10% DMSO
Mycoplasma Tested: Yes

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