pF3K WG plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pF3K WG plasmid vector质粒载体

: The pF3K WG (BYDV) Flexi® Vector(a–d) is designed for use with the Flexi® System, Transfer (Cat.# C8820),
and the Flexi® System, Entry/Transfer (Cat.# C8640). The vector contains translation enhancer (TE) sequences from the barley
yellow dwarf virus (BYDV), an RNA plant virus, that facilitate in vitro expression of protein in wheat germ extract. T7 and SP6
RNA polymerase promoters allow production of RNA for subsequent translation applications. Linear DNA templates for in vitro
RNA transcription can be produced by cutting at one of the restriction enzyme sites located downstream of the 3´ BYDV TE
region. The vector also contains the lethal barnase gene for positive selection of the insert, a kanamycin-resistance gene for
selection of the plasmid in E. coli and unique SgfI and PmeI sites, which allow easy insertion or transfer of the sequence of
interest. Inserts containing a protein-coding region can easily be transferred from the pF3K WG (BYDV) Flexi® Vector to other
Flexi® Vectors with different expression options
pF3K WG (BYDV) Flexi® Vector Features and Circle Map
pF3K WG (BYDV) Flexi® Vector sequence reference points:
SP6 RNA polymerase promoter (–17 to +3) 23–42
T7 RNA polymerase promoter (–17 to +1)* 46–63
BYDV 5´ UTR 65–201
SgfI site 202–209
barnase coding region 233–568
PmeI site 570–577
BYDV 3´ TE 596–702
T7 terminator 807–854
kanamycin resistance gene 1235–2029
origin of replication 2198–2234
cer site (site for E. coli XerCD recombinase) 2905–3190
rrnB transcription terminator 3241–3642
*Note: The consensus T7 RNA polymerase promoter extends only to +1 and does not match
many standard T7 promoter primers available for sequencing applications. However, the SP6
Promoter Primer (Cat.# Q5011) can be used to sequence inserts cloned into the pF3K WG
(BYDV) Flexi® Vector


Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net