pVALIUM20 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pVALIUM20 plasmid vector质粒载体

pVALIUM20
Feature type engineered_plasmid Component Allele(s)
Size 8.257
pVALIUM20 allows cloning of short hairpins as annealed synthetic oligos, targeted against specific genes via the production of short hairpin microRNAs; contains v+t1.8 as a selectable marker; the hsp70 basal promoter; a multiple cloning site (MCS) for cloning shRNAs in a mir-1 scaffold; a ftz 3'UTR intron followed by a SV40 3'UTR to facilitate shRNA processing and export from the nucleus; two pentamers of UAS, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; two gypsy insulator sequences flanking the transcription unit to enhance hairpin DNA transcription; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.

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