NubI-Alg5 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：NubI-Alg5
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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NubI-Alg5 plasmid vector质粒载体
Halo-bioassay (agonist-induced growth arrest assay) for STE20-, STE11- and STE2-gene-disrupted strains: NMY51 (WT); NMY61 (ste20Δ); NMY62 (ste11Δ); and NMY63 (ste11Δ ste2Δ). Each paper filter disk was spotted with the indicated amount of α-factor. (B) Growth assay of NMY51 (WT; a,b), NMY61 (ste20Δ; c,d) and NMY62 (ste11Δ; e,f) strains on SD –Leu, Trp, Ade and His dropout plates. Yeast strains harboring pBT3-C/pPR3-C or pCCW-Alg5/pAI-Alg5 respectively expressed Cub/NubG (negative control; a,c,e) or Alg5-Cub/Alg5-NubI (positive control; b,d,f). Each cell was spotted in serial 10-fold dilutions on selective agar plates with or without 5 µM of α-factor. NubI is a WT Nub tag and interacts spontaneously with Cub.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Halo-bioassay (agonist-induced growth arrest assay) for STE20-, STE11- and STE2-gene-disrupted strains: NMY51 (WT); NMY61 (ste20Δ); NMY62 (ste11Δ); and NMY63 (ste11Δ ste2Δ). Each paper filter disk was spotted with the indicated amount of α-factor. (B) Growth assay of NMY51 (WT; a,b), NMY61 (ste20Δ; c,d) and NMY62 (ste11Δ; e,f) strains on SD –Leu, Trp, Ade and His dropout plates. Yeast strains harboring pBT3-C/pPR3-C or pCCW-Alg5/pAI-Alg5 respectively expressed Cub/NubG (negative control; a,c,e) or Alg5-Cub/Alg5-NubI (positive control; b,d,f). Each cell was spotted in serial 10-fold dilutions on selective agar plates with or without 5 µM of α-factor. NubI is a WT Nub tag and interacts spontaneously with Cub.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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