Influenza B virus乙型流感病毒毒株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Influenza B virus乙型流感病毒毒株

Product category
Viruses
Product type
Animal virus
Classification
Orthomyxoviridae, Influenzavirus B
Strain designation
B/Taiwan/2/62
Type strain
No
Isolation source
Patient.
Geographical isolation
Taiwan
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Specific applications
Emerging infectious disease research
Respiratory research
Influenza Research
Comments
Derived by adaptation from allantoic product NTCC® VR-295™ to MDCK cells (NTCC® CCL-34™). NTCC® VR-295™ and NTCC® VR-1735™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture.
Mycoplasma contamination
Not detected
Host
MDCK (NBL-2) (NTCC CCL-34)
Effects
cell degeneration; cell rounding; CPE
Complete medium
EMEM (NTCC® 30-2003™) + 10 mM HEPES + 0.125% BSA fraction V + 1 µg/mL TPCK-treated Trypsin
Temperature
33°C
Atmosphere
95% Air, 5% CO2
Incubation
2-4 days
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Key abbreviations
°C, Degrees Celsius
BSA, Bovine serum albumin
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
MOI, Multiplicity of infection
TPCK, L-1-tosylamido-2-phenylethyl chloromethyl ketone
Volume
1.0 mL

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