N2a-APP695swe NTCC®小鼠神经母细胞瘤细胞基因稳转株 cell line BioVector NTCC质粒载体菌种细胞基因保藏中心

N2a-APP695swe NTCC®小鼠神经母细胞瘤细胞基因稳转株 cell line BioVector NTCC质粒载体菌种细胞基因保藏中心

A transgenic mouse N2a neuroblastoma cells that stably express wild-type or mutated APP, respectively. C-terminally myc-tagged wild-type APP, or APP carrying the Swedish familial Alzheimer's disease (FAD)-specific amino acid substitution were cloned in plasmid pCB6 under control of CMV promoter. Next, mouse N2a neuroblastoma cells were transfected with either construct and stabilized using antibiotic treatment. Presence of recombinant protein was validated via anti-myc immunoblotting and immunoprecipitation.

The stable cell lines will allow researchers to examine the intracellular processing of wild-type or mutated APP and the production of Ab peptides, the principal component of amyloid plaques in Alzheimer's disease.

N2a-APP695swe is a cell line derived from mouse neuroblastoma cells (N2a) that have been genetically engineered to express a mutant form of the amyloid precursor protein (APP) known as APP695swe. This mutation, the Swedish mutation, is linked to familial Alzheimer's disease and results in increased production of amyloid-beta (Aβ) peptides, a key hallmark of the disease.

This cell line is widely used in Alzheimer's disease research for several reasons:

• Modeling Aβ Production: It provides a convenient model system to study the mechanisms of Aβ production and aggregation.

• Drug Screening: It can be used to screen for potential therapeutic compounds that may inhibit Aβ production or promote its clearance.

• Understanding Disease Mechanisms: It allows researchers to investigate the cellular and molecular pathways involved in Alzheimer's disease pathogenesis.

By studying the N2a-APP695swe cell line, researchers can gain valuable insights into the complex mechanisms underlying Alzheimer's disease and identify potential targets for therapeutic intervention.

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