Bacillus megaterium , strain MS941 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：Bacillus megaterium , strain MS941
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Bacillus megaterium , strain MS941
The pretransformed Bacillus megaterium protoplast, strain MS941, is part of the T7 RNA Polymerase high yield gene expression system for Bacillus megaterium. This B. megaterium protoplast comes pretransformed with pT7-RNAP so that the gene of interest can be inserted into pPT7 immediately. Please note: this protoplast has a limited shelf life of a few months from the date of production. About the T7 RNA polymerase expression system The Bacillus megaterium high yield T7 gene expression system combines the features of the B. megaterium system in E. coli with the T7 RNA polymerase expression system regulation by the xylose operon. This dual plasmid system is based on two parallel-replicating plasmids: pT7-RNAP and pPT7. pT7-RNAP contains the genes for ampicillin and chloramphenicol resistance for easy selection in E. coli (AmpR) and B. megaterium (CmR), in addition to the t7 rnap gene under control of the strong xylA promoter. pPT7 is responsible for the T7 RNAP-dependent expression of the target gene. Downstream of the T7 promoter, it comprises a multiple cloning site with ten unique restriction enzyme cleaving sites. Additionally, pPT7 comprises two resistances against ampicillin (in E. coli) and tetracycline (in B. megaterium). B. megaterium has proven to be an excellent host for the expression of non-homologous DNA. Unlike other bacilli strains, none of the alkaline proteases are present and no endotoxins are found in the cell wall. B. megaterium can stably maintain several extra-chromosomal DNA elements in parallel. Protein yields are exceptionally high, even if inexpensive substrates are used.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The pretransformed Bacillus megaterium protoplast, strain MS941, is part of the T7 RNA Polymerase high yield gene expression system for Bacillus megaterium. This B. megaterium protoplast comes pretransformed with pT7-RNAP so that the gene of interest can be inserted into pPT7 immediately. Please note: this protoplast has a limited shelf life of a few months from the date of production. About the T7 RNA polymerase expression system The Bacillus megaterium high yield T7 gene expression system combines the features of the B. megaterium system in E. coli with the T7 RNA polymerase expression system regulation by the xylose operon. This dual plasmid system is based on two parallel-replicating plasmids: pT7-RNAP and pPT7. pT7-RNAP contains the genes for ampicillin and chloramphenicol resistance for easy selection in E. coli (AmpR) and B. megaterium (CmR), in addition to the t7 rnap gene under control of the strong xylA promoter. pPT7 is responsible for the T7 RNAP-dependent expression of the target gene. Downstream of the T7 promoter, it comprises a multiple cloning site with ten unique restriction enzyme cleaving sites. Additionally, pPT7 comprises two resistances against ampicillin (in E. coli) and tetracycline (in B. megaterium). B. megaterium has proven to be an excellent host for the expression of non-homologous DNA. Unlike other bacilli strains, none of the alkaline proteases are present and no endotoxins are found in the cell wall. B. megaterium can stably maintain several extra-chromosomal DNA elements in parallel. Protein yields are exceptionally high, even if inexpensive substrates are used.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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