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E. coli ER2267 strain大肠杆菌菌株
A suspension of E. coli ER2267 which has been grown in rich medium and brought to 50% glycerol. MM294 background: EcoK r– m+ McrA– McrBC– Mrr– . Genotype: F´ proA+ B+ lacIq Δ(lacZ)M15 zzf::miniTn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14– (McrA– ) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10Restriction Defects: McrA, McrBC and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (3,4). Restriction by each of these three restriction systems individually (tested with l DNA modified with the M.Sss I phage) is: McrA, 200-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is > 5,000-fold. In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites(5). ER2267 is deficient in all four systems. Other Properties: Selectable F´ (Kanr ); suitable for use with M13, pUC-type, and hybrid (phagemid) vectors that make use of lac α-fragment complementation (blue/white screen); overproduces lac repressor (lacI q ); recA stabilizes repeat sequences and reduces plasmid multimerization.References: 1.Kelleher and Raleigh (1991) J. Bact. 173, 5220–5223. 2.2. Raleigh and Wilson (1986) PNAS 83, 9070–9074.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
A suspension of E. coli ER2267 which has been grown in rich medium and brought to 50% glycerol. MM294 background: EcoK r– m+ McrA– McrBC– Mrr– . Genotype: F´ proA+ B+ lacIq Δ(lacZ)M15 zzf::miniTn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14– (McrA– ) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10Restriction Defects: McrA, McrBC and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (3,4). Restriction by each of these three restriction systems individually (tested with l DNA modified with the M.Sss I phage) is: McrA, 200-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is > 5,000-fold. In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites(5). ER2267 is deficient in all four systems. Other Properties: Selectable F´ (Kanr ); suitable for use with M13, pUC-type, and hybrid (phagemid) vectors that make use of lac α-fragment complementation (blue/white screen); overproduces lac repressor (lacI q ); recA stabilizes repeat sequences and reduces plasmid multimerization.References: 1.Kelleher and Raleigh (1991) J. Bact. 173, 5220–5223. 2.2. Raleigh and Wilson (1986) PNAS 83, 9070–9074.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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